EFFICACY TEST OF ETANOL EXTRACT OF TALLBOOD LEAVES (Nicotiana tabacum L.) AS A BIOLARVASIDA AGAINST AEDES AEGYPTI LARVAE.
Keywords:
Dengue Fever, Lethal Time and Lethal concentration, Aedes aegypti, Biolarvicide, Mortality.Abstract
DHF is an infectious disease caused by the entry of the dengue virus into the body through the bite of the Aedes aegypti mosquito that appears throughout the year. Climate change causes changes in rainfall, temperature, humidity and air direction that affect health, especially in the development of disease vectors such as the aedes mosquito. Tobacco leaves are useful as larvicides because they contain alkanoids, saponins, polyphenols, flavonoids, steroids, and quinones. The higher the concentration of tobacco leaf extract, the higher the larvicidal effect because nicotine or alkaloids function as a poison that can affect the digestive system and nervous system of larvae while other compounds can function to damage the respiratory and gastrointestinal systems. This study aims to identify the potential of ethanol extract of tobacco leaves as a biolarvicide against dengue fever. This study used a completely randomized design (CRD) with each concentration tested in stages, namely 12.5%, 25%, and 50% to obtain LC50.95 and LT50.95 Probit regression and linear regression were analyzed using the SPSS application. In this study there were three treatment groups, each group had 3 repetitions with concentrations of 12.5%, 25%, and 50% respectively. Each replicate was observed in minutes for 24 hours. The results obtained at a concentration of 50% the highest larval mortality rate with a percentage of 30.7%, 25% concentration with a percentage of 10.7%, then at a concentration of 12.5% with a percentage of 0%.
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